Background and Goals Synthetic Model Complexes Photoelectron Spectroscopy and Density Functional Theory Electron Paramagnetic Resonance Biochemical Studies People


Enemark Research Group

Biochemical Studies

Subcloning of mSO Gene Into pQE-60 Expression Vector

Expression of Sulfite Oxidase from Mus musculus

Sequence Comparison of the Mo Domains of Sulfite Oxidases
(Red) conserved residues, (blue) similar, (black) not similar

Mutagenesis Studies on Mouse Sulfite Oxidase
  • Investigate and study charged residues on the surface of SO.
  • Study mutations in the loop region of SO.
  • Investigate mutations that cause SO deficiency.

Oxidation State Changes in Animal SO During Catalysis

Photochemically Induced 1-Electron Reduction of SO and Subsequent Intramolecular Electron Transfer (IET)

 

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