Electron Paramagnetic Resonance

We use variable-frequency pulsed EPR techniques to explore the electronic structure and coordination geometry of Mo(V) sulfite oxidase intermediates and of isotopically-labeled Mo(V) model compounds. Both the ESEEM and HYSCORE spectra, shown below, allow for detection of the weakly coupled axial 17O in the active site of SO.

Ka band (~29.3 GHz) 4-pulse ESEEM spectra of [Mo17O(SPh)4]- and hpH chicken SO in H217O obtained at gz.

hpH SO/H217O: HYSCORE at gy (10632 G) νmw = 29.28 GHz