MASS SPECTROMETRY FACILITY UA Dept of Chemistry
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Sample Preparation: Trypsin Digestion of Proteins in Solution Sample clean-up and trypsin digestion in solution HTML PDF Sample Preparation: Trypsin Digestion of In-Gel Proteins Following are some general protocols for the enzymatic
digestion of proteins separated by 1-D or 2-D electrophoresis. Please
note that samples vary dramatically, and conditions (materials, methods &
reagents) must be optimized for individual sample types. Silver Stained Gels (with destaining) Silver Stained Gels (without destaining) Coomassie Stained Gels Clean-up of digested proteins by Zip Tip Suggestions for obtaining intact protein from gels
(caution: low yield) HTML
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