Ghosh Lab Publications

Selected Publications

Stains, C.I.; Furman, J.L.; Porter, J.R.; Rajagopal, S.; Li, Y.; Wyatt, R.T.; Ghosh, I.; "A General Approach for Receptor and Antibody-Targeted Detection of Native Proteins Utilizing Split-Luciferase Reassembly" ACS Chem. Biol., 2010, Article ASAP. [Reprint] | [Supporting Information]

Furman, J.L.; Badran, A.H.; Ajulo, O.; Porter, J.R.; Stains, C.I.; Segal, D.J.; Ghosh, I.; "Toward a General Approach for RNA-Templated Hierarchical Assembly of Split Proteins" J. Am. Chem. Soc., 2010, 132 (33), 11692-11701. [Reprint] | [Supporting Information]

Jester, B.W.; Cox, K.J.; Gaj, A.; Shomin, C.D.; Porter, J.R.; Ghosh, I.; "A Coiled-Coil Enabled Split-Luciferase Three-Hybrid System for Profiling Inhibitors of Protein Kinases" J. Am. Chem. Soc., 2010, 132 (33), 11727-11735. [Reprint] | [Supporting Information]

Shekhawat, S.S.; Porter, J.R.; Sripasad, A.; Ghosh, I.; "An Autoinhibited Coiled-Coil Design Strategy for Split-Protein Protease Sensors" J. Am. Chem. Soc., 2009, 131 (42), 15284-15290. [Reprint] | [Supporting Information]

Shomin, C.D.; Meyer, S.C.; Ghosh, I.; "Staurosporine tethered peptide ligands that target cAMP-dependent protein kinase (PKA): Optimization and selectivity profiling" Bioorg. Med. Chem., 2009, 17 (17), 6196-6202. [Reprint] | [Supporting Information]

Furman, J.L.; Badran, A.H.; Shen, S.Y.; Stains, C.I.; Hannallah, J.; Segal, D.J.; Ghosh, I.; "Systematic evaluation of split-fluorescent proteins for the direct detection of native and methylated DNA" Bioorg. Med. Chem. Lett., 2009, 19 (14), 3748-3751. [Reprint] | [Supporting Information]

Porter, J.R.; Stains, C.I.; Jester, B.W.; Ghosh, I.; "A General and Rapid Cell-Free Approach for the Interrogation of Protein−Protein, Protein−DNA, and Protein−RNA Interactions and their Antagonists Utilizing Split-Protein Reporters" J. Am. Chem. Soc., 2008, 130 (20), 6488–6497. [Reprint] | [Supporting Information]

Stains, C.I.; Mondal, K.; Ghosh, I.; "Molecules that Target beta-Amyloid," ChemMedChem, 2007, 2 (12), 1675-1692. [Reprint] (Review)

Meyer, S.C.; Shomin, C.D.; Gaj, T.; Ghosh, I.; "Tethering Small Molecules to a Phage Display Library: Discovery of a Selective Bivalent Inhibitor of Protein Kinase A," J. Am. Chem. Soc., 2007, 129 (45), 13812 - 13813. [Reprint] | [Supporting Information]

Porter, J. R.; Stains, C. I.; Segal, D. J.; Ghosh, I.; "Split beta-Lactamase Sensor for the Sequence-Specific Detection of DNA Methylation," Anal. Chem., 2007, 79 (17), 6702-6708. [Reprint]

Gaj, T.; Meyer, S.C.; Ghosh, I.; "The AviD-tag, a NeutrAvidin/avidin specific peptide affinity tag for the immobilization and purification of recombinant proteins," Protein. Expres. Purif., 2007, 56 (1), 54-61. [Reprint]

Stains, C.I.; Ghosh, I.; "When conjugated polymers meet amyloid fibrils," ACS Chem. Biol., 2007, 2 (8), 525-528. [Reprint] (Point of View)

Ghosh, I.; Wirth M.J.; "Parsing the Motion of Single Molecules: A Novel Algorithm for Deconvoluting the Dynamics of Individual Receptors at the Cell Surface," Science's STKE, 2007, 388, 28. (Perspectives)

Zhou, M.; Nakatani, E.; Gronenberg, L.S.; Tokimoto, T.; Wirth, M.J.; Hruby, V.J.; Roberts, A.; Lynch, R.M.; Ghosh, I; "Peptide-Labeled Quantum Dots for Imaging GPCRs in Whole Cells and as Single Molecules," Bioconjugate Chem., 2007, 18 (2), 323 -332. [Reprint]

Tokimoto, T.; Bethea, T.R.C.; Zhou, M.; Ghosh, I.; Wirth M.J.; "Probing orientations of single fluorescent labels on a peptide reversibly binding to the human delta-opioid receptor," Applied Spectroscopy, 2007, 61 (2), 130-137.

Zhou, M.; Ghosh, I.; "Quantum Dots and Peptides: A Bright Future Together," Peptide Science, 2007, 88, 325-339. [Reprint] (Review)

Smith, T.J. ; Stains, C.I.; Meyer, S.C.; Ghosh, I.; "Inhibition of beta-Amyloid Fibrillization by Directed Evolution of a beta-Sheet Presenting Miniature Protein," J. Am. Chem. Soc. 2006, 128, 14456-14457. [Reprint] | [Supporting Information]

Ghosh, I; Stains, C.I.; Ooi, A.T.; Segal, D.J.; "Direct detection of double-stranded DNA: molecular methods and applications for DNA diagnostics," Mol. BioSyst., 2006, 2, 551-560. [Reprint] (Review)

Rajagopal, S.; Meyer, S.C.; Goldman, A.; Zhou, M.; Ghosh, I.; "A Minimalist Approach towards Protein Recognition by Epitope Transfer from Functionally Evolved Beta-Sheet Surfaces," J. Am. Chem. Soc. 2006, 128, 14356-14363. [Reprint] | [Supporting Information]

Stains, C.I.; Furman, J.L.; Segal, D.J.; Ghosh, I.; "Site Specific Detection of DNA Methylation Utilizing mCpG-SEER," J. Am. Chem. Soc., 2006, 128, 9761 - 9765. [Reprint] | [Supporting Information]

Meyer, S.C.; Gaj, T.; Ghosh, I.; "Highly Selective Cyclic Peptide Ligands for NeutrAvidin and Avidin Identified by Phage Display," Chemical Biology and Drug Design, 2006, 68, 3-10. (cover, September 2006) [Reprint] | [Supporting Information]

Ooi, A.T.; Stains, C.I.; Segal, D.J.; Ghosh, I.; "Sequence-Enabled Reassembly of beta-Lactamase (SEER-LAC): A Sensitive Method for the Detection of Double-Stranded DNA," Biochemistry, 2006, 45, 3620 -3625. [Reprint] | [Supporting Information]

Stains, C.I.; Porter, J.R.; Ooi, A.T.; Segal, D.J.; Ghosh, I.; "DNA Sequence-Enabled Reassembly of the Green Fluorescent Protein," Journal of the American Chemical Society; 2005, 127, 10782 -10783. [Reprint] | [Supporting Information]
Research Highlight: "DNA Makes GFP Shine," Nature Methods, 2005, 2, 644.

Meyer, S.C.; Huerta, C; Ghosh, I.; "Single-Site Mutations in a Hyperthermophilic Variant of the B1 Domain of Protein G Result in Self-Assembled Spherical Oligomers," Biochemistry; 2005, 44, 2360 -2368. [Reprint] | [Supporting Information]

Ghosh, I.; Chmielewski, J.; "Peptide self-assembly as a model of proteins in the pre-genomic world," Current Opinion in Chemical Biology; 2004, 8, 640-644. [Reprint] (Review)

Zhou, M.; Ghosh, I.; "Noncovalent Multivalent Assembly of Jun Peptides on a Leucine Zipper Dendrimer Displaying Fos Peptides," Organic Lett.; 2004, 6, 3561 -3564. [Reprint] | [Supporting Information]

Rajagopal, S.; Meza-Romero, R.; Ghosh, I.; "Dual Selection Methodology for the Identification of Thrombin Binding Epitopes from Hotspot Biased Phage-Display Libraries," Bioorg. & Med. Chem. Lett.; 2004, 14, 1389-1393. (cover) [Reprint]

Zhou, M.; Bently, D.; Ghosh, I.; "Helical Supramolecules and Fibers Utilizing Leucine-Zipper Displaying Dendrimers," J. Am. Chem. Soc., 2004, 126, 734-735. [Reprint] | [Supporting Information-1] | [TEM Images]
Highlighted in Chemical and Engineering News Science Concentrates 2004, 82 (04), 41.

Magliery, T.J.; Wilson, C.G.M.; Pan, W.; Mishler, D.; Ghosh, I.; Hamilton, A.D.; Regan, L.; "Detecting Protein-Protein Interactions with a Green Fluorescent Protein Fragment Reassembly Trap: Scope and Mechanism," J. Am. Chem. Soc., 2005, 127, 146-157.

Ghosh, I; Hamilton, A. D.; Regan, L.; "Selection of protein-protein interactions by split GFP in living cells," US Patent Application, 2002.

Ghosh, I; Bishop, P.; Chmielewski, J.; " DNA binding properties of basic-helix-loop-helix fusion proteins of Tal and E47," J. Pept. Res., 2001, 57, 354-360.

Ghosh, I; Hamilton, A. D.; Regan, L. "Leucine Zipper Assisted Protein Reassembly: Application to the Green Fluorescent Protein," J. Am. Chem. Soc., 2000, 122, 5658-5659.

Ma, Y. S.; Cunningham, M. E.; Ghosh, I; Regan, L.; Longley, B. J.; "Inhibition of spontaneous receptor phosphorylation by residues in a putative alpha-helix in the KIT intracellular juxtamembrane region," J. Biol. Chem., 1999, 274, 13399-13402.

Ghosh, I; Issac, R.; Chmielewski, J.; "Structure-function relationship in a beta-sheet peptide Inhibitor of E47 Dimerization and DNA Binding," Bioorg. Med. Chem., 1999, 7, 61-66.

Ghosh, I.; Chiemelewski, J.; "Ab- Sheet Inhibitor of IEB E47-Dimerization and DNA Binding," Chem. Biol., 1998, 5, 439-445.

Yao, S.; Ghosh, I.; Zutshi, R.; Chmielewski, J.; "Selective Amplification via Auto- and Cross-catalysis in a Replicating Peptide System," Nature, 1998, 396, 447-450.

Yao, S.; Ghosh, I.; Chmielewski, J.; "Natural Selection in Self-Replicating Peptides", Peptides: Chemistry, Structure and Biology, 1998, 15, 0000.

Yao, S.; Ghosh, I.; Zutshi, R.; Chmielewski, J.; "Self-replicating Peptide under Ionic Control," Angew. Chem. Int. Ed. Eng., 1998, 37, 478-481.

Ghosh, I.; Yao, S.; Chmielewski, J.; " DNA Binding Peptides," Comprehensive Natural Products Chemistry, 1998, 7, 477-490.

Yao, S.; Ghosh I.; Zutshi, R.; Chmielewski, J.; "A pH-Modulated Self-Replicating Peptide," J. Am. Chem. Soc., 1997, 119, 10559-10560.

Bishop, P.; Ghosh, I.; Jones, C.; Chmielewski, J.; "The Basic-Helix-Loop-Helix Region of Tal: Evaluation of Structure and DNA Affinity," J. Am. Chem. Soc., 1995, 117, 8283.