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Visuals for Bruker DRX-600 NMR Training Workshop Lecture II

These visuals are made from the original lecture transparencies

Two Dimensional (2D) NMR

2-D Spectrum: a matrix of data values

Display of 2D Data

Transfer of Magnetization: Mixing

General Pulse Sequence for 2D Experiments

Diagram of evolution during t₁ and t₂ periods: nth t₁ value

Diagram of evolution during t₁ and t₂ periods: (n+1)th t₁ value

Diagram of evolution during t₁ and t₂ periods: (n+2)th t₁ value

Diagram of evolution during t₁ period

2D Data Processing

2D Data Matrix: after F₂ Fourier transform

2D Spectrum: after F₁ Fourier transform

Summary of 2D NMR Experiments

2D Connections (Shift Correlation)

2D Correlation of ¹H with ¹³C: HETCOR

HETCOR (Heteronuclear Correlation)

2-D HETCOR: diagram for transfer of magnetization

HETCOR spectrum of sucrose

2D Correlation of ¹H with ¹³C: HSQC / HMQC

Comparison of HMQC and HETCOR

HMQC / HSQC: overview

2D Correlation of ¹H with ¹³C: HMBC

HMQC/HSQC vs. HMBC

Cartoon of 2D HSQC and HMBC spectra without ¹³C decoupling

HMQC Spectrum of 3-Heptanone without ¹³C Decoupling

HMQC spectrum of sucrose in D₂O : no ¹³C decoupling

HMQC spectrum of sucrose in D₂O : no ¹³C decoupling, with ¹J_CH values

HMQC of sucrose: non-anomeric CH region

HMQC of cholesterol without ¹³C decoupling: methyl region

HSQC Experiment: What Happens When

HSQC pulse sequence (simplified)

Using Gradients to Improve the HSQC

Gradient-Enhanced HSQC: 2 gradient pulses

Edited Decoupled HSQC

¹³C-decoupled HSQC of sucrose in D₂O (500 MHz, gradients)

Decoupled Edited HSQC of Testosterone Metabolite "product 2"

HSQC of Testosterone Metabolite "product 2": upfield region

HSQC of Testosterone Metabolite "product 2": upfield region with assignments

HSQC of Testosterone Metabolite "product 2": upfield region with stereospecific assignments